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+ # powerhouse.scroll.pub

+

+ slideshow generated by Claude from prompt: Project PowerHouse Presentation Slide 1: Title Slide Title: Project PowerHouseSubtitle: Build Your Own At-Home Mitochondria Imaging LabPresenter: Breck YunitsDate: January 9, 2025 Slide 2: Introduction Who We Are: Biohacking initiative to democratize mitochondrial imaging. Vision: Build the world's largest public domain database of mitochondria images. Why Mitochondria? Every human has approximately 30 trillion cells, each with about 1,000 mitochondria. Central to cellular energy, signaling, and health. Slide 3: The Problem Current Challenges in Mitochondria Imaging: High Costs: Access to fluorescence microscopes and reagents is expensive. Limited Access: Most data resides in private or institutional silos. Fragmented Resources: No central repository of high-quality, public domain images. Slide 4: The Solution Project PowerHouse: Create an affordable, reproducible home lab setup for mitochondrial imaging. Empower global collaboration to build a public domain mitochondria database. Slide 5: How It Works Components of the Setup: Microscope: Affordable fluorescence microscopes or DIY solutions. Reagents: Stains like Rhodamine 123. Image Analysis Software: Mitocounter.py (Open source). Data Sharing Platform: ScrollSet for standardized data collection. Slide 6: Mitocounter Demo Input: Image of stained mitochondria.Output: Count and analysis of mitochondrial population. Live Demo or Screenshots: Example input image. Example output visualization. Slide 7: Cost Breakdown Lab Equipment Costs: Bench fees: $1,500/month (discounted for first year). Shared equipment access: $490/month per user. DIY Alternatives: Fluorescence microscopes from AmScope starting at $1,000. Rhodamine 123: $116 for 50mg from Biotium. Slide 8: Collaboration Opportunities Why Partner with Us? Democratize mitochondrial research. Expand access to biohacking tools. Accelerate the creation of open databases for global scientific discovery. Next Steps: Join as a lab partner or advisor. Contribute to the database or software development. Slide 9: Roadmap January 2025: Secure lab space and finalize equipment procurement. March 2025: Begin data collection and community outreach. June 2025: Public launch of the mitochondria image database. Ongoing: Develop partnerships and enhance imaging tools. Slide 10: Conclusion Key Takeaways: Mitochondria imaging is critical to understanding health and disease. Project PowerHouse aims to make this accessible to everyone. Together, we can build a resource that transforms biohacking and research. Contact Information: Breck Yunits Website | Twitter Slide 11: Q&A Questions?Let’s discuss how you can be part of Project PowerHouse!

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